平台编号：bio-74689
细胞信息：U-2 OS
规格：frozen
用途：ATCC原装细胞
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注意事项：仅用于科学研究或者工业应用等非医疗目的不可用于人类或动物的临床诊断或治疗，非药用，非食用（产品信息以出库为准）
U-2 OS
U-2 OS
(ATCC® HTB-96™)
Disease: osteosarcoma
Organism 微生物 Homo sapiens, human
Tissue bone
Product Format 提供形式 frozen
Morphology epithelial Culture Properties adherent
Biosafety Level 生物安全等级 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease osteosarcoma Age 15 years Gender female Ethnicity Caucasian
Applications This cell line is a suitable transfection host.
Karyotype Cell line U-2 OS is chromosomally highly altered, with chromosome counts in the hypertriploid range.
We did not find the hypodiploid cell population described by J. Ponten, et al., Instead, most of the population has slightly higher counts than first described. Very few normal chromosomes are present, but a high number of stable marker chromosomes are identified., Different chromosomal rearrangements involving the same chromosomes (N1, N7, N9, and N11 particularly), are seen.
Twenty-two markers are found including: t(9qter--->9q21::1p36--->1p::?), 7p+, iso(17q), t(15q;?), 4q+, del(3)(q21), 5q(aberrant) and others. Ref
Images Derivation J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma of the tibia of a 15 year old girl.
Clinical Data 15 years Caucasian female J. Ponten and E.
Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma of the tibia of a 15 year old girl.
Antigen Expression Antigen expression: Blood Type A; Rh+; HLA A2, Aw30, B12, Bw35, B40(+/-) Receptor Expression
Receptor expression: insulin-like growth factor I (IGF-I); insulin-like growth factor II (IGF II) Genes
Expressed osteosacoma derived growth factor (ODGF),Blood Type A; Rh+; HLA A2, Aw30, B12, Bw35, B40(+/-)
Comments 注释 Viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses.
Mycoplasma contamination was detected and eliminated in 1972.
Complete Growth Medium The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007.
To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution.
Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution.
Allow the flask to sit at room temperature (or at 37℃) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: 2 to 3 times per week Cryopreservation Culture medium, 95%; DMSO, 5%
Culture Conditions 培养条件
Temperature 培养温度 : 37℃
STR Profile Amelogenin: X CSF1PO: 13 D13S317: 13 D16S539: 11,12 D5S818: 11 D7S820: 11,12 THO1: 6,9.3 TPOX: 11,12 vWA: 14,18 Isoenzymes AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 PGM1, 2 PGM3, 1
Name of Depositor Hellstrom Year of Origin 1964
References 参考文献 Heldin CH, et al. A human osteosarcoma cell line secretes a growth factor structurally related to a homodimer of PDGF A-chains. Nature 319: 511-514, 1986. PubMed: 3456080
Ponten J, Saksela E. Two established in vitro cell lines from human mesenchymal tumours. Int. J. Cancer 2: 434-447, 1967. PubMed: 6081590
Raile K, et al. Human osteosarcoma (U-2 OS) cells express both insulin-like growth factor-I (IGF-I) receptors and insulin-like growth factor-II/mannose-6- phosphate (IGF-II/M6P) receptors and synthesize IGF-II: autocrine growth stimulation by IGF-II via the IGF-I receptor. J. Cell. Physiol. 159 : 531-541 , 1994. PubMed: 8188767
Landers JE, et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562-3568, 1997. PubMed: 9270029
Moradpour D, et al. Characterization of cell lines allowing titghtly regulated expression of heapatitis C virus core protein. Virology 222: 51-63, 1996. PubMed: 8806487
Ponten J, Saksela E. Two established in vitro cell lines from human mesenchymal tumours. Int. J. Cancer 2: 434-447, 1967. PubMed: 6081590
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