Organism Homo sapiens, human
Tissue skin Cell Type fibroblast
Product Format frozen
t Culture Properties adherent
Biosafety Level 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age 12 week gestation
Storage Conditions liqudi nitrogen vapor phase
Derivation WS1 was derived from normal human skin by RJ Hay.
Clinical Data 12 week gestation Black female
Comments WS1 cells have a doubling potential of 67 population doublings.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003.
To make the complete growth medium,
add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
2，Remove and discard culture medium.
2，Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
3，Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37℃ to facilitate dispersal.
4，Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
5，Add appropriate aliquots of the cell suspension to new culture vessels.
6，Incubate cultures at 37℃.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: Twice per week
Cryopreservation Freeze medium: culture medium, 95%; DMSO, 5% Storage temperature: liqudi nitrogen vapor phase
Culture Conditions Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37℃