运输方式:冻存运输
器官来源:脾
数量:大量
细胞形态:淋巴样
ATCC Number:CRL-9300?
细胞类型:B淋巴细胞
是否是肿瘤细胞:0
物种来源:人
生长状态:悬浮生长
规格:0.5mg Designations: 20H11
Depositors: ?Genetic Systems Corp.
Biosafety Level:2 [Cells Contain HERPESVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Organ: spleen
Cell Type: B lymphocyte; Epstein-Barr virus (EBV) transformed
Cellular Products:immunoglobulin; monoclonal antibody; against Pseudomonas aeruginosa (flagella type b)
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Comments:Peripheral blood B cells from a patient immunized with high molecular weight polysaccharide from P. aeruginosa were transformed with Epstein-Barr virus.
The antibody reacts with P aeruginosa Fisher immunotypes 2, 6 and 7.
The antibody cross-reacts extensively with clinical isolates of P. aeruginosa bearng type b flagella.
Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium with 2 mM L-glutamine, 85%; heat-inactivated fetal bovine serum, 15%
Subculturing: Medium Renewal: Every 3 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Doubling Time: 24 to 36 hrs
References: 3871: Rosok MJ, Lostrom ME. Monoclonal antibodies to Pseudomonas aeruginosa flagella. US Patent 4,834,976 dated May 30 1989