运输方式:冻存运输
生长状态:悬浮生长
数量:大量
器官来源:外周血
年限:33 years
细胞形态:淋巴样
免疫类型:IgM; lambda light chain
ATCC Number:CCL-156?
细胞类型:B淋巴细胞
是否是肿瘤细胞:0
物种来源:人
规格:1mg Designations: RPMI 1788
Depositors: ?GE Moore
Isotype: IgM; lambda light chain
Biosafety Level:2 [Cells Contain HERPESVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Organ: peripheral blood
Cell Type: B lymphocyte;
Cellular Products:immunoglobulin; lymphotoxin (LT) also known as tumor necrosis factor beta (TNF-beta, TNF beta)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:HLA A2, Aw33, B7, B14
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 10
D13S317: 11,13
D16S539: 10,13
D5S818: 12,13
D7S820: 10,12
THO1: 6,9.3
TPOX: 8,9
vWA: 18,19
Cytogenetic Analysis:human male; hypodiploid; stable
Isoenzymes: G6PD, B
Age: 33 years
Gender: male
Ethnicity: Caucasian
Comments:The cells are EBNA positive.
Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10% - OR - RPMI 1640 medium, 80%; fetal bovine serum, 20%
Subculturing: Medium Renewal: Add fresh medium (10% to 20% by volume) every 3 to 4 days
Cultures can be maintained by addition or replacement of fresh medium.Cultures are established at 3 X 10 exp5 viable cells/ml.
References: 22796: Aggarwal BB, et al. Human lymphotoxin. Production by a lymphoblastoid cell line, purification, and initial characterization. J. Biol. Chem. 259: 686-691, 1984. PubMed: 6608523
22918: Gray PW, et al. Cloning and expression of cDNA for human lymphotoxin, a lymphokine with tumour necrosis activity. Nature 312: 721-724, 1984. PubMed: 6334807
23213: Huang CC, Moore GE. Chromosomes of 14 hematopoietic cell lines derived from peripheral blood of persons with and without chromosome anomalies. J. Natl. Cancer Inst. 43: 1119-1128, 1969. PubMed: 5353241