器官来源：脾
生长状态：悬浮生长
ATCC Number：HB-9580?
是否是肿瘤细胞：0
物种来源：小鼠
细胞形态：淋巴样
免疫类型：mouse IgG2a kappa
年限：adult
运输方式：冻存运输
品系：BALB/c
数量：大量
规格：0.5mg Designations: R6.5.D6.E9.B2 [R6-5-D6]
Depositors: ?Dana Farber Cancer Inst.
Isotype: mouse IgG2a kappa
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast


Source: Organ: spleen
Strain: BALB/c
Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against human intracellular adhesion molecule 1 (ICAM-1)
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Age: adult
Comments:Animals were immunized with the EBV transformed lymphoblast cell line, JY.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody binds to ICAM-1 and inhibits the interaction of ICAM-1 with leukocytes.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10(4) viable cells/ml. Do not allow the cell concentration to reach 6 X 10(5) cells/ml.
Interval: Maintain cultures at a cell concentration between 3 X 10(4) and 5 X 10(5) cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with extra 10% FBS and 7.5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 12 hours
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 22051: Springer TA, et al. Intercellular adhesion molecules, and their binding ligands. US Patent 5,284,931 dated Feb 8 1994