ATCC Number:CRL-1996?
数量:大量
细胞形态:淋巴样
免疫类型:IgG1
是否是肿瘤细胞:0
物种来源:小鼠
运输方式:冻存运输
生长状态:悬浮生长
规格:0.2ml Designations: KL295
Depositors: ?JA Frelinger
Isotype: IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against the beta chain of mouse Class II antigens
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Applications:Animals were immunized with synthetic peptide corresponding to residues 146 to 157 of the mouse Ab/beta protein (domain beta 1).
The antibody reacts with denatured beta chains of the b, d, p, q, j and bu haplotypes.
ATCC uses ultra-low IgG fetal bovine serum (Invitrogen catalog # 16250).
Comments:Animals were immunized with synthetic peptide corresponding to residues 146 to 157 of the mouse Ab/beta protein (domain beta 1).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with denatured beta chains of the b, d, p, q, j and bu haplotypes.
It does not detect the intact H-2b Class II molecule, and does not detect the beta chain on the cell surface.
It does not react with molecules of the k, f, and s haplotypes.
The reactive determinant is most likely involves residues 153 through 155.
Depositor recommends using low IgG serum. ATCC uses ultra-low IgG fetal bovine serum (Invitrogen catalog # 16250).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 1% nonessential amino acids, 10 mM HEPES, 0.01 mM 2-mercaptoethanol and 10% fetal bovine serum
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22809: LaPan KE, et al. Production and characterization of two new mouse monoclonal antibodies reactive with denatured mouse class II beta chains. Hybridoma 11: 217-223, 1992. PubMed: 1376718