细胞形态:淋巴样
免疫类型:IgG2b
数量:大量
运输方式:冻存运输
品系:BALB/c
ATCC Number:CRL-2859?
生长状态:悬浮生长
器官来源:脾
是否是肿瘤细胞:0
物种来源:小鼠
规格:0.1ml Designations: CBRM1/1
Depositors: ?TA Springer
Isotype: IgG2b
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus
Morphology:lymphoblast
Source: Strain: BALB/c
Organ: spleen
Cell Type: hybridoma: lymphoblast B lymphocyte; somatic cell hybrid
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions:Please acknowledge the origin of this cell line in all relevant publications by citing the following publication(s): [89838 ] [89839 ]
Isolation: Isolation date: November 19, 1991
Comments:Mice (BALB/c) were immunized with purified Mac1 (CD11b/CD18). Spleen cells were fused with P3X63Ag8.653 (ATCC CRL-1580 ). Monoclonal antibodies from selected hybridomas recognized the Mac-1 alpha subunits on the surface of neutrophils. [Pubmed: 7679388] The monoclonal antibodies produced by these hybridomas will be useful in determining the function of Mac-1 subregions involved in ligand recognition. [Pubmed: 9614126].
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(4) to 1 X 10(5) viable cells/ ml. Maintain cultures at a cell concentration between 2 X 10(5) and 1 X 10(6) cells/ml.
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 89838: Lu C, et al. The structure of the beta-propeller domain and C-terminal region of the integrin alphaM subunit. Dependence on beta subunit association and prediction of domains.. J. Biol. Chem. 273: 15138-15147, 1998. PubMed: 9614126
89839: Diamond MS, et al. The I domain is a major recognition site on the leukocyte integrin Mac-1 (CD11b/CD18) for four distinct adhesion ligands. J. Cell Biol. 120: 1031-1043, 1993. PubMed: 7679388