ATCC® Number:PCS-100-011
Name: Primary Aortic Endothelial Cells; Normal, Human
Biosafety Level:1
Shipped: frozen
Organism: Homo sapiens (human)
Source: Aortic endothelium
Growth Properties: Adherent
Age: Adult
Morphology: Cobblestone appearance
Number of Viable Cells per Vial: ≥ 500,000 in cryopreservation medium
Volume per Vial: 1 ml
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Related Products:Vascular Cell Basal Medium (ATCCPCS-100-030)Endothelial Cell Growth Kit-BBE (ATCCPCS-100-040)Endothelial Cell Growth Kit-VEGF (ATCCPCS-100-041)Dulbecco's Phosphate Buffered Saline (D-PBS) (ATCC30-2200)Trypsin-EDTA for Primary Cells (ATCCPCS-999-003)Trypsin Neutralizing Solution (ATCCPCS-999-004)Gentamicin-Amphotericin B Solution (ATCCPCS-999-025)Penicillin-Streptomycin-Amphotericin B Solution (ATCCPCS-999-002)Phenol Red (ATCCPCS-999-001)
Comments:A complete solution to propagate Aortic Endothelial cells in low serum conditions with or without the addition of human recombinant VEGF. Use of the Endothelial Cell Growth Kit-VEGF will support a faster rate of proliferation because of the presence of several purified human recombinant (rh) growth factors (rh VEGF, rh EGF, rh FGF basic and rh IGF-1) combined with heparin and hydrocortisone. Use of the Endothelial Cell Growth Kit-BBE, which contains Bovine Brain Extract (BBE), is recommended if a more authentic, less defined culture medium is desired.
Applications:Arteriolosclerosis, hypertension, arterial disease, vascular wound healing, inflammation, tissue remodeling, vascular differentiation, apoptosis, drug screening, stent development.
Viability:≥ 50%
Population Doubling Capacity:≥ 15 in complete growth medium
Sterility Tests:Bacteria and Yeast: Negative
Mycoplasma: Negative

Testing:Hepatitis B: Negative Hepatitis C: Negative HIV-1: Negative