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Aspergillus niger van Tieghem 拉丁名

(ATCC 16888 ) 统一编号

Strain Designations 别名 WB 326 [CBS 554.65, IMI 50566, NRRL 326; 2766] 

Application 用途 Produces aniline ；Produces gluconic acid； Produces glucose oxidase ；Produces glucosidase, beta; acid Glucosidase, beta, acid, glucocerebrosidase； Produces kynureninase-type enzymes ；Produces pergolide sulfone； Produces pergolide sulfoxide； Produces pseudonigeron (1-3)-alpha-D-glucan ；Degrades: pulp and paper-mill wastewater ；Fermentation of tannin-gallic acid ；Transformation of sesquiterpene lactone costunolide ；Transformation of acetanilide to aniline ；Transformation of pergolide to pergolide sulfoxide and pergolide sulfone 

Biosafety Level 生物安全等级 1 

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. 

Product Format 提供形式 freeze-dried 

Storage Conditions 保藏条件

Frozen 冷冻物: -80℃ or colder 

Freeze-Dried 冻干物: 2℃ to 8℃ 

Live Culture: See Propagation Section 

Type Strain 模式菌种 yes (Al-Musallam A Revision of the black Aspergillus species, 1980) 

Preceptrol® yes 

Comments 注释 Aspergillus reference strain for identification and morphological observation recommended by the International Commission on Penicillium and Aspergillus (ICPA) Structure of galactomannan 

Medium 培养基 ATCC Medium 325: Malt extract agar (Blakeslee's formula) 

ATCC Medium 28: Emmons' modification of Sabouraud's agar

ATCC Medium 336: Potato dextrose agar (PDA) 

Growth Conditions

Temperature: 24℃ to 26℃ 

Atmosphere: Typical aerobic 

Sequenced Data 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence

GGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGGTCCTTTGGGCCCAACCTCCCATCCGTGTC

TATTGTACCCTGTTGCTTCGGCGGGCCCGCCGCTTGTCGGCCGCCGGGGGGGCGCCTCTGCCCCCCGGGCCCG

TGCCCGCCGGAGACCCCAACACGAACACTGTCTGAAAGCGTGCAGTCTGAGTTGATTGAATGCAATCAGTTA

AAACTTTCAACAATGGATCTCTTGGTTCCGGCATCGATGAAGAACGCAGCGAAATGCGATAACTAATGTGAAT

TGCAGAATTCAGTGAATCA

TCGAGTCTTTGAACGCACATTGCGCCCCCTGGTATTCCGGGGGGCATGCCTGTCCGAGCGTCATTGCTGCCC

TCAAGCCCGGCTTGTGTGTTGGGTCGCCGTCCCCCTCTCCGGGGGGACGGGCCCGAAAGGCAGCGGCGGCACC

GCGTCCGATCCTCGAGCGTATGGGGCTTTGTCACATGCTCTGTAGGATTGGCCGGCGCCTGCCGACGTTTTCCA

ACCATTCTTTCCAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAA

D1D2 region of the 28S ribosomal RNA gene

ATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATTGCCTCAGTAACGGCGAGTGAAGCGGCAAGA

GCTCAAATTTGAAAGCTGGCTCCTTCGGAGTCCGCATTGTAATTTGCAGAGGATGCTTTGGGTGCGGCCCCC

GTCTAAGTGCCCTGGAACGGGCCGTCAGAGAGGGTGAGAATCCCGTCTTGGGCGGGGTGTCCGTGCCCGTG

TAAAGCTCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAATGGGTGGTAAATTTCATCTAAAGCT

AAATACTGGCCGGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGAGAGTTA

AACAGCACGTGAAATTGT

TGAAAGGGAAGCGCTTGCGACCAGACTCGCCCGCGGGGTTCAGCCGGCATTCGTGCCGGTGTACTTCCCCGTG

GGCGGGCCAGCGTCGGTTTGGGCGGCCGGTCAAAGGCCCCTGGAATGTAGTGCCCTCCGGGGCACCTTATAGCC

AGGGGTGCAATGCGGCCAGCCTGGACCGAGGAACGCGCTTCGGCACGGACGCTGGCATAATGGTCGTAAACGAC

Beta-tubulin (bTub)

TTGCCCTCCCCGTCCCTCGTCCGTCAGGAGACGCGTCGTTGGTTGGCATCTCTTTTGCTCGGGACCCCACCGG

TTCTTCGACCAACTCATTCTTGTGCTAACTGCATGTCTTCTTCGCTTCATAGGTTCACCTCCAAACCGGCCAGTGTGTAAGTG

CCAATATATGCTTCGGATGATTGCCCCCAAGGGTCTTGATTGGTGTTTGGTGGACTAAACAATATATCATGGTGGTTAGG

GTAACCAAATTGGTGCTGCTTTCTGGTACGTATACAACTGCCATTGGATTGGGGATGGAACATCGTCTCTTAGGCTATCTCAGCTT

GAGTTCAGATGTTGTCCATTAGGTACATGCTATCGGTCTAAGAACACGTCTAACAATTCAACAGGCAGACCATCTCTGGCGAGC

ACGGCCTTGACGGCTCCGGTGTGTAAGTGCAACTTTTTCACACCTCTCAATTGGTCAACAATGGGCAAAGGGTTGGGTCTTCTG

ACACGCAGGATAGTTACAATGGCACCTCCGACCTCCAGCTGGAGCGCATGAACGTCTACTTCAACGAGGTGAGATCCATCGG

ACCTTGGCTTTTTCACGACAATATCATCAATGTCCTAATCACTTCAGCAGGCTAGCGGTAACAAGTATGTTCCTCGTGCCGTCCTC

GTCGACCTCGAGCCCGGTACCATGGACGCCGTCCGTGCCGGTCCTTTCGGCCAGCTCTTCCGCCCCGACAACTTCGTCTTCGG

CCAGTCCGGTGCTGGTAACAACTGG

Name of Depositor寄存者 KB Raper, DI Fennell

Chain of Custody 来源国家 ATCC <-- KB Raper, DI Fennell <-- NRRL 326 <-- C Thom 2766 <-- A Hollander

Isolation分离基物 Not available.

Cross ReferencesNucleotide (GenBank) : KU729129 D1/D2 region of 28S rRNA gene

Nucleotide (GenBank) : FJ195350 ITS including 5.8S rRNA gene

References 参考文献 Storm AM, Gasner LL. Treatment of pulp mill wastes. US Patent 3,737,374 dated Jun 5 1973

Klich MA, et al. Aspergillus reference cultures. New Orleans and Baarn: International Commission on Penicillium and Aspergillus; 1996.

Al-Musallam A Revision of the black Aspergillus species, 1980

Raper KB, Fennell DI. The genus Aspergillus. Baltimore: Williams & Wilkins; 1965.

Bardalaye PC, Nordin JH. Chemical structure of the galactomannan from the cell wall of Aspergillus niger. J. Biol. Chem. 252: 2584-2591, 1977. PubMed: 856795

Lasswell WL, et al. An undergraduate experiment in pharmacognosy: the microbial biotransformation of acetanilide by Aspergillus species. Am. J. Pharm. Educ. 47: 127-129, 1983.

Clark AM, Hufford CD. Microbial transformations of the sesquiterpene lactone costunolide. J. Chem. Soc. Perkin Trans. 1979: 3022-3028, 1979.

Smith RV, et al. Microbial transformations of pergolide to pergolide sulfoxide and pergolide sulfone. J. Pharm. Sci. 72: 733-736, 1983. PubMed: 6684155

Horisberger M, et al. Structure of A (1--->3) -alpha-D-glucan (pseudonigeron) of Aspergillus niger NRRL 326 cell wall. Carbohydr. Res. 23: 183-188, 1972. PubMed: 4340387

Witte K, Wartenberg A. Purification and properties of two beta-glucosidases isolated from Aspergillus niger. Acta Biotechnol. 9: 179-190, 1989.

Murado MA, et al. Enhancement of the bioproduction potential of an amylaceous effluent. Bioresour. Technol. 44: 155-163, 1993.

Shetty AS, Gaertner FH. Kynureninase-type enzymes of Penicillium roqueforti, Aspergillus niger, Rhizpus stolonifer, and Pseudomonas fluorescens: Further evidence for distinct kynureninase and hydroxykynureninase activities. J. Bacteriol. 122: 235-244, 1975. PubMed: 164432

FF MicroPlate. Biolog.

Lawrence DP, et al. Interkingdom gene transfer of a hybrid NPS/PKS from bacteria to filamentous Ascomycota. PLoS One 6: e28231, 2011. PubMed: 22140558

Andersen MR, et al. Comparative genomics of citric-acid-producing Aspergillus niger ATCC 1015 versus enzyme-producing CBS 513.88. Genome Res. 21: 885-897, 2011. PubMed: 21543515

Yamada O, et al. Molecular biological researches of Kuro-Koji molds, their classification and safety. J. Biosci. Bioeng. 112: 233-237, 2011. PubMed: 21641278

Peterson SW. Phylogenetic analysis of Aspergillus species using DNA sequences from four loci. Mycologia 100: 205-226, 2008. PubMed: 18595197

Geiser DM, et al. The current status of species recognition and identification in Aspergillus. Stud Mycol 59: 1-10, 2007. PubMed: 18490947

Serra R, et al. Aspergillus ibericus: a new species of section Nigri isolated from grapes. Mycologia 98: 295-306, 2006. PubMed: 16894975

Bau M, et al. DNA-based characterization of ochratoxin-A-producing and non-producing Aspergillus carbonarius strains from grapes. Res. Microbiol. 156: 375-381, 2005. PubMed: 15808942Shimoda M, et al. Accelerated death kinetics of Aspergillus niger spores under high-pressure carbonation. Appl. Environ. Microbiol. 68: 4162-4167, 2002. PubMed: 12147527

Henry T, Iwen PC, Hinrichs SH. Identification of Aspergillus species using internal transcribed spacer regions 1 and 2. J. Clin. Microbiol. 38: 1510-1515, 2000. PubMed: 10747135

Perrone G, et al. Ochratoxin A production and amplified fragment length polymorphism analysis of Aspergillus carbonarius, Aspergillus tubingensis, and Aspergillus niger strains isolated from grapes in Italy. Appl. Environ. Microbiol. 72: 680-685, 2006. PubMed: 16391107