ATCC® Number:VR-302™
Classification: Poxviridae, Orthopoxvirus
Agent: Cowpox virus deposited as Cowpox virus, Orthopoxvirus
Strain: Brighton
Original Source: Lesions on hands of milker, England, 1937
Depositors: AW Downie
Biosafety Level:2
Shipped: frozen
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Host Organism : rabbit, i. d. inoculation; primary rabbit kidney cells rabbit; chicken embryo (chorioallantoic membrane); chicken embryo cells
Incubation : Duration: 2-3 days
HostBS-C-1 (ATCC CCL-26)
Effectslocalized hemorrhagic lesion at inoculation site
Complete mediumLeibovitz's L-15 (ATCC® 30-2008™) + 2% FBS (ATCC® 30-2020™).
Temperature37°C
Atmosphere95% Air, 5% CO2
Incubation2-3 daysRecommendation for infectionPlant cells 24-48 hours in advance and infect when cultures are 80-90% confluent.
Remove medium and inoculate with a small volume (e.g. 1 mL per 25 cm2) of virus diluted with VGM to provide a MOI of about 0.1-1.0.
Adsorb 1 hour at 37°C in a humidified 5% CO2 atmosphere.
End adsorption by adding virus growth medium.Handling notesThis strain is recognized as prototype strain by Virus Subcommittee of the International Nomenclature Committee (Fenner A, Burnet, FM, 1957).
Key abbreviationsBS-C-1, African green monkey kidney cells
°C, Degrees Celsius
CO2, Carbon dioxide
CPE, Cytopathic effect
FBS, Fetal bovine serum
MOI, Multiplicity of infection
Effect : Yes, in vivo effects: Localized hemorrhagic lesion at inoculation site
Comments : Handle as potentially biohazardous material under Biosafety Level 2+ containment. Virus characteristics are essentially the same as vaccinia. REFERENCE STRAIN: Fenner, F. et al., Virology 4: 305, 1957. GENBANK AF043237, Cowpox virus natural killer cell receptor gene, complete cds. GENBANK X75158, Cowpox virus gene for 14kDa fusion protein Vaccination recommended for lab staff manipulating vaccinia, monkeypox, or cowpox viruses (CDC/NIH Biosafety in Microbiological and Biomedical Laboratories, 4th ed, 1999 - stock no. 017-040-00547-4). Essentially same as vaccinia.
References : 33475: Davies JH. . Lancet 235: 1534, 1938. 33476: Downie AW. . Br. J. Exp. Pathol. 20: 158, 1939.