ATCC® Number:VR-838™
Classification: Poxviridae, Orthopoxvirus
Agent: Raccoonpox virus deposited as Raccoonpox virus, Orthopoxvirus
Strain: Herman
Original Source: Isolated by Y.F. Herman from respiratory tract of raccoon with no clinical symptoms, Maryland, USA, 1964
Depositors: JH Nakano
Biosafety Level:2
Shipped: frozen
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Host Organism : LLC-MK2 cells (ATCCCCL-7); Vero (ATCCCCL-81) Vero cells (ATCCCCL-81); LLC-MK2 cells (ATCCCCL-7); CAM; suckling mouse
Incubation : Temperature: 35.5°C Duration: 3-4 days
Effect : Yes, in vitro effects: Cytopathic effects (large plaques and giant cell syncytia) in Vero cells Yes, in vivo effects: paralysis in suckling mouse Yes, in vivo effects: pinpoint pocks on chorioallantoic membranes
Comments : The virus does not grow well on chorioallantoic membranes after 2 to 3 passages. The virus cross-reacts serologically with the Connaught strain of vaccinia virus. Raccoons inoculated with RPV show no apparent clinical symptoms; however, their sera demonstrate strong HAI reactions. Does not grow well on CAM after 2-3 passages. Cross-reacts with Connaught strain on vaccinia virus. Raccoons inoculated with RPV show no apparent clinical symptoms; however, their sera demonstrate strong HAI reactions.
References : 33948: Thomas EK, et al. Further characterization of Raccoonpox virus. Arch. Virol. 49: 217-222, 1975. PubMed: 813616 33949: Herman YF. Bact. Proc. 64th Annual Meeting, ASM : 117, 1964.