ATCC® Number:VR-3125™
Classification: Poxviridae, Orthopoxvirus
Agent: Vaccinia virus deposited as Vaccinia virus, Orthopoxvirus
Strain: IHD-W Dts28
Depositors: S Dales, R. Condit
Biosafety Level:2 [BSL 2 Vaccination recommended for lab staff mipulating vaccinia, monkeypox, or cowpox viruses ]
Shipped: frozen
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Host Organism : Production Host: BSC-40 (ATCCCRL-2761) Alternate Host(s): MRC-5, BS-C-1, HeLa, LLC-MK2
Incubation : 1-3 days at 31 C with 5% CO2
Effect : Cytopathic effects in cell culture include cell rounding and death, with eventual sloughing
Store at : -70 C or lower
Comments : Selected after mutagenesis with nitrosoguanidine. The non-permissive incubation temperature is 39.5 C. This mutant was assigned a Dales EM categories G and P, characterized by incomplete membranes with spicules, immature normal particles and mature particles with cores that are predominantly lucent at the center. The mutation was assigned by complementation analysis to vaccinia map location A24, the 132-kDa viral RNA polymerase subunit. [70405]
Related Products: VR-1441