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首页 / 美国ATCC / ATCC病毒类 / Chlamydia trachomatis
Chlamydia trachomatis
Chlamydia trachomatis
  • 平台编号:bio-49462
  • 拉丁属名:Chlamydia trachomatis
  • 规格:frozen
  • 用途:ATCC原装进口
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  • 注意事项:仅用于科学研究或者工业应用等非医疗目的不可用于人类或动物的临床诊断或治疗,非药用,非食用(产品信息以出库为准)

详细信息

Chlamydia trachomatis 拉丁名 

(ATCC® VR-1477™) 统一编号

Classification Chlamydiaceae, Chlamydia 

Deposited As Chlamydia trachomatis (Busacca) Rake 

Agent Chlamydia trachomatis 

Strain TW-3 [Trachoma type C strain TW-3] 

Applications 用途 Sexually Transmitted Disease Research Positive for Mycoplasma Yes 

Biosafety Level 生物安全等级 2 

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. 

Product Format 提供形式 frozen 1 mL per vial 

Storage Conditions 保藏方法 -70°C or colder 

Comments 注释 FBS used to culture Chlamydia must be prescreened to verify that the serum does not contain antibodies to Chlamydia or other factors that would interfere with growth. 

This strain is a tissue culture-adapted version of strain TW-3 (VR-578) which is grown in embryonated chicken eggs. 

Before infecting host cells, add 1-mm glass beads and vortex stock material for 45 seconds. Aspirate fluid. Infect shell vial monolayer with 0.2 mL of material. Centrifuge at 800 x G for 1 hour. 

Feed with 1.0 mL EMEM (HEPES buffered), 10% FBS (screened for chlamydia antibodies). 

Serotype C Effect on Host Requires FA to visualize intracellular inclusion bodies 

Recommended Host HEp-2 cells (ATCC® CCL-23™) 

McCoy cells (ATCC® CRL-1696™) 

Growth Conditions 生长条件 

Temperature 培养温度 : 37°C 

Recommendations for Infection: Add glass beads and vortex preparation to disrupt cells. 

Infect monolayer with disrupted material. Centrifuge at 3000 x rpm (750 x g) for 1 hour. 

Feed with fresh growth medium containing FBS prescreened for Chlamydia antibodies and 1-2mg/mL cycloheximide. 

Incubate at 37°C for 48 to 72 hours, a 5% CO2 in air atmosphere is recommended. 

Incubation: 48 to 72 hours, a 5% CO2 in air atmosphere is recommended. 

Effect on Host Requires FA to visualize intracellular inclusion bodies 

Name of Depositor 寄存人 Kuo 

Special Collection ATCC Source Specimen from human conjunctiva, Taiwan 

References 参考文献 Grayston JT, et al. Trachoma-studies of etiology, laboratory diagnosis, and prevention. JAMA 172: 1577-1586, 1960. PubMed: 13829148