ATCC® Number:VR-1363™
Classification: Rickettsiae
Agent: Rickettsia japonica Uchida et al.
Strain: YH
Original Source: blood of patient with oriental spotted fever, Japan
Depositors: T Uchida
Biosafety Level:3
Shipped: frozen
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Host Organism : Vero cells (ATCCCCL-81) BHK-21(C-13) cells (ATCCCCL-10); L-929 cells (ATCCCCL-1); chicken embryo; guinea pig
Incubation : Temperature: 34.0°C Duration: 3-6 days
Effect : Yes, in vitro effects: Cytopathic effects in BHK and L929 Yes, in vitro effects: No observable CPE in Vero Yes, in vivo effects: death of CE Yes, in vitro effects: fever and scrotal swelling of guinea pig
Comments : Species-specific monoclonal antibody only reacts with R. japonica but not with other spotted fever group rickettsiae. Vero cells demonstrated to be mycoplasma-free before infection. Infected Vero cells grow like uninfected cells in MEM with 10% FBS devoid of antibiotics. Subculture by dispersion with 0.05% trypsin and 0.02% EDTA is possible for many passages. Restrictions: This material is not available for international distribution due to the lack of historical data to support the issuance of a U.S. Fish and Wildlife Service Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES) export permit.
References : 11731: Uchida T, et al. Rickettsia japonica sp. nov., the etiological agent of spotted fever group rickettsiosis in Japan. Int. J. Syst. Bacteriol. 42: 303-305, 1992. PubMed: 1581190 11774: Uchida T, et al. Isolation of a spotted fever group Rickettsia from a patient with febrile exanthematous illness in Shikoku, Japan. Microbiol. Immunol. 30: 1323-1326, 1986. PubMed: 3553872