ATCC® Number:50386™
Organism: Spironucleus vortens Poynton et al.
Isolation: lip lesion, angelfish, Pterophyllum scalare, Archer, FL (29 degrees 32' N, 82 degrees 23' W), 1991
Depositors: SL Poynton
Biosafety Level:1
Shipped: frozen
Growth Conditions: Temperature: 35.0°C Duration: axenic; anaerobic Protocol: ATCCNO: 50386 SPEC: This culture is shipped as a frozen stabilate (0.5 ml of cell suspension frozen in a Nunc 1.2 ml conical bottom plastic screw-capped vial). Thaw the ampule according to instructions accompanying the shipment. Add entire suspension to 13 ml of ATCCmedium 1141. Screw the cap on tightly and incubate the tube at 25C on a horizontal slant (5-15 degrees). Transfer the culture weekly; invert the tube 5-10 times, aseptically transfer a 0.1 ml aliquot to a fresh tube of medium, and incubate as above.
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Type Strain: yes [20916]
Cross References: Nucleotide (GenBank) : U93086 Spironucleus vortens small subunit rRNA gene, copy 2, partial sequence.Nucleotide (GenBank) : U94406 Spironucleus vortens elongation factor-1 alpha gene, partial cds.Nucleotide (GenBank) : U94408 Spironucleus vortens translation initiation factor-2 gamma subunit gene, partial cds.Nucleotide (GenBank) : U94407 Spironucleus vortens alpha-tubulin gene, partial cds.Nucleotide (GenBank) : U93085 Spironucleus vortens small subunit rRNA gene, copy 1 , partial sequence.
References: 20916: Poynton S, et al. Spironucleus vortens n. sp. from the freshwater angelfish Pterophyllum scalare: Morphology and culture. J. Eukaryot. Microbiol. 42: 731-742, 1995. 16172712: Dawson SC, et al. Stable transformation of an episomal protein-tagging shuttle vector in the piscine diplomonad Spironucleus vortens. BMC Microbiol. 8:71, 2008. PubMed: 18445284.