ATCC® Number:PRA-134™
Organism:
Rosculus sp.
Designations: BSA-02190017
Isolation: sediment, saltwater (sampled from a sparina area; Catleshed Marsh (near Hog Island), eastern shore of Virginia)Virginia, United States
Depositors: JC Cole, TA Nerad
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 1525: Seawater 802 mediumTemperature: 20.0°C Duration: xenic; Klebsiella pneumoniae subsp. pneumoniae (ATCC700831) and as a food source Protocol:
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Scrape the bottom of the flask, quickly agitate and withdraw 0.2 ml of cell suspension.
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Inoculate into 10 ml of ATCCmedium 1525 bacterized with Klebsiella pneumoniae subsp. pneumoniae (ATCC700831).Note: the amoebae settle very quickly, so the transfer must be done very quickly to be effective.
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Incubate the flask at 20C.
Interval: biweekly
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