【成份】
1059 ANAEROCELLUM MEDIUM
酵母提取物 (Difco) 0.3 g
Na2-succinate 1.0 g
(NH4)-acetate 0.5 g
Fe(III) citrate solution (0.1% in H2O) 5.0 ml
KH2PO4 0.5 g
MgSO4 x 7 H2O 0.4 g
NaCl 0.4 g
NH4Cl 0.4 g
CaCl2·2 H2O 0.05 g
Vitamin B12 solution (10 mg in 100 ml H2O) 0.4 mlg
Trace element solution SL-10(见培养基1064) 1.0 ml
L-Cysteiniumchloride 0.3 g
Resazurin(0
1%) 0.5 ml
蒸馏水 1.0 L
Adjust pH to 6.8
Boil the medium for a few minute. Bubble the medium with nitrogen gas and fill 10 ml in 15 ml tubes with a rubber septum under a stream of nitrogen gas. Autoclave at 121C for 15 min. Sterile syringes are used to inoculate and remove samples. Incubate in the light using a tungsten lamp.