【成份】
1054 CALDICELLULOSIRUPTOR MEDIUM
NH4Cl 0.9 g
NaCl 0.9 g
MgCl2·6 H2O 0.4 g
MgCl2 7.0 g
K2HPO4 1.5 g
KH2PO4 0.75 g
Trypticase peptone 2.0 g
酵母提取物 (Difco) 1.0 g
Trace element solution SL-10(见培养基1064) 1.0 ml
FeCl3·6 H2O solution (0.1% w
in 0.2 N HCl) 2.5 ml
Na-resazurin solution (0.1% w
) 0.5 ml
L-Cysteine-HCl·H2O 0.75 g
Cellobiose 1.0 g
蒸馏水 1.0 L
Dissolve ingredients except cysteine and cellobiose. Sparge medium with 100% N2 gas for 30 – 45 min to make it anoxic
then add cysteine and adjust pH to 7.2. Distribute medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add cellobiose after autoclaving from an anoxic stock solution prepared under 100% N2 gas atmosphere and sterilized by filtration. Adjust pH of the complete medium to 7.2
if necessary.