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Homo sapiens (human)
  • 平台编号:bio-69648
  • 规格:0.1ml
  • 拉丁属名:
  • 购买数量:
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是否是肿瘤细胞:0
物种来源:人
ATCC Number:PCS-100-022
生长状态:贴壁生长
细胞形态:其他
运输方式:冻存运输
年限:Lot-specific
数量:大量
规格:0.1ml Name: Primary Pulmonary Artery Endothelial Cells; Normal, Human
Biosafety Level:1
Shipped: frozen
Organism: Homo sapiens (human)
Source: Pulmonary artery
Growth Properties: Adherent
Age: Lot-specific
Morphology: Cobblestone appearance
Volume per Vial: 1 ml
Ethnicity: Lot-specific
Gender: Lot-specific
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Related Products:Primary Pulmonary Artery Smooth Muscle Cells; Normal, Human (ATCC PCS-100-023 )
Endothelial Cell Growth Kit-BBE (ATCC PCS-100-040 )
Endothelial Cell Growth Kit-VEGF (ATCC PCS-100-041 )
Dulbecco's Phosphate Buffered Saline (D-PBS) (ATCC 30-2200 )
Trypsin-EDTA for Primary Cells (ATCC PCS-999-003 )
Trypsin Neutralizing Solution (ATCC PCS-999-004 )
Gentamicin-Amphotericin B Solution (ATCC PCS-999-025 )
Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002 )
Phenol Red (ATCC PCS-999-001 )
Recomended Culture Conditions:See Product Information Sheet Comments:The cells are cryopreserved in the third passage to ensure the highest purity, coupled with high viability and plating efficiency.
A complete solution to propagate pulmonary artery endothelial cells in low serum conditions with or without the addition of human recombinant VEGF. Use of the Endothelial Cell Growth Kit-VEGF will support a faster rate of proliferation because of the presence of several purified recombinant human (rh) growth factors (rh VEGF, rh EGF, rh FGF basic and rh IGF-1) combined with heparin and hydrocortisone. Use of the Endothelial Cell Growth Kit-BBE, which contains Bovine Brain Extract (BBE), is recommended if a more "authentic," less defined culture medium is desired.
Applications:Angiogenesis, arteriolosclerosis, hypertension, oncology, drug screening, inflammation, vasodilator function.
Viability:≥ 50%
Population Doubling Capacity:≥ 15 in complete growth medium
Sterility Tests: Bacteria and Yeast: Negative
Mycoplasma: Negative

Testing: Hepatitis B: Negative
Hepatitis C: Negative
HIV-1 and HIV 2: Negative
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