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MES-SA/Dx5
  • 平台编号:bio-69093
  • 规格:60 次×3
  • 拉丁属名:
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生长状态:贴壁生长
细胞形态:成纤维样
年限:56 years
器官来源:子宫
运输方式:冻存运输
数量:大量
ATCC Number:CRL-1977?
相关疾病:其他疾病
是否是肿瘤细胞:1
物种来源:人
规格:60 次×3 Designations: MES-SA/Dx5
Depositors: ?BI Sikic
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast


Source: Organ: uterus
Disease: uterine sarcoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
DNA Profile (STR):Amelogenin: X
CSF1PO: 11
D13S317: 13
D16S539: 11,12
D5S818: 13
D7S820: 7,11
THO1: 6
TPOX: 8,11
vWA: 18
Cytogenetic Analysis:modal number = 47; the karyotype is 47XX, 12q+, 22q-, t(5q,6p), -3, -4, -7, +t(3q?,7q), Mar 1, + Mar 2, + Mar 3; the two additional marker chromosomes indicate clonal selection during drug selection
Age: 56 years
Gender: female
Ethnicity: Caucasian
Comments:The multiple drug resistant cell line MES-SA/Dx5 was established from MES-SA cells (ATCC CRL-1976 ) which were grown in the presence of increasing concentrations of doxorubicin.
The cells exhibit a marked cross resistance to a number of chemotherapeutic agents including vinblastine, taxol, colchicine, vincristine, etoposide, dactinomycin, mitoxantrone and daunorubicin.
They exhibit moderate cross resistance to mitomycin C and melphalan.
Resistance to bleomycin, cisplatin, carmustine, 5-fluorouracil or methotrexate was not observed.
The cells express high levels of mdr-1 mRNA and P-glycoprotein.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Growth Conditions: Newborn calf serum may be substituted for fetal bovine serum.
Subculturing: Protocol: Remove spent medium, add fresh EDTA solution (0.15 g disodium EDTA, 4.0 g NaCL, 0.28 g sodium bicarbonate, 0.5 g dextrose and 0.2 g KCl dissolved in 500 ml double distilled water). Allow the cells to sit at room temperature for a few minutes, and dislodge the cells by rapping the side of the flask sharply with the palm of your hand. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Remove spent medium, add fresh EDTA solution (0.15 g disodium EDTA, 4.0 g NaCL, 0.28 g sodium bicarbonate, 0.5 g dextrose and 0.2 g KCl dissolved in 500 ml double distilled water).
Allow the cells to sit at room temperature for a few minutes, and dislodge the cells by rapping the side of the flask sharply with the palm of your hand.
Add fresh medium, aspirate and dispense into new flasks.
Doubling Time: 30 hrs
Related Products:parental cell line:ATCC CRL-1976
References: 23048: Harker WG, et al. Development and characterization of a human sarcoma cell line, MES-SA, sensitive to multiple drugs. Cancer Res. 43: 4943-4950, 1983. PubMed: 6883344
23058: Harker WG, Sikic BI. Multidrug (pleiotropic) resistance in doxorubicin-selected variants of the human sarcoma cell line MES-SA. Cancer Res. 45: 4091-4096, 1985. PubMed: 4028002
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