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LLC-MK2 Original
  • 平台编号:bio-69012
  • 规格:0.5mg
  • 拉丁属名:
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是否是肿瘤细胞:0
物种来源:猕猴
ATCC Number:CCL-7?
相关疾病:正常
数量:大量
生长状态:贴壁生长
细胞形态:上皮样
年限:adult
运输方式:冻存运输
器官来源:肾脏
规格:0.5mg Designations: LLC-MK2 Original
Depositors: ?RN Hull
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Macaca mulatta
Morphology:epithelial


Source: Organ: kidney
Disease: normal
Cellular Products:plasminogen activator
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: adult
Comments:The serum used in the medium should be pre-tested for good quality.
Some lots of horse serum do support satisfactory growth.
Adaptation to other types of sera generally results in slow growth with a prolonged lag in growth.
Propagation: ATCC complete growth medium: Medium 199 (with 1.68 g/L bicarbonate), 99%; horse serum, 1%
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Centrifuge the cell suspension at 1000 rpm for 10 minutes, resuspend the pellet in fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products:recommended serum:ATCC 30-2040
References: 22310: . . Anat. Rec. 124: 490, 1956.
26049: Hull RN, et al. Growth characteristics of monkey kidney cell strains LLC-MK1, LLC-MK2, and LLC-MK2(NCTC-3196) and their utility in virus research. J. Exp. Med. 115: 903-918, 1962. PubMed: 14449901
26065: Hull RN, Tritch OJ. Characterization of cell strains by viral susceptibility. Natl. Cancer Inst. Monogr. 7: 161-172, 1962. PubMed: 14449902
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