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2D12
  • 平台编号:bio-68844
  • 规格:0.5mg
  • 拉丁属名:
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数量:大量
生长状态:悬浮生长
细胞形态:淋巴样
免疫类型:IgG2a; kappa light chain
ATCC Number:CRL-1689?
运输方式:冻存运输
是否是肿瘤细胞:0
物种来源:小鼠
规格:0.5mg Designations: 2D12
Depositors: ?JJ Schlesinger
Isotype: IgG2a; kappa light chain
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against yellow fever virus (vaccine strains and Asibi strain)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:H-2d
Comments:Mice were immunized with the 17D strain of yellow fever virus.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody does not cross-react with other flaviviruses.
Antibody reactivity can be assayed by hemagglutination inhibition, complement fixation and immunofluorescence.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: RPMI 1640 medium containing non-essential amino acids, 20 mM HEPES, 1 mM sodium pyruvate, 2 mM L-glutamine, and 0.02 mM 2-mercaptoethanol, 85%; fetal bovine serum, 15%

Subculturing: Medium Renewal: Every 3 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Maintain cell concentration between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%
References: 1014: Schlesinger JJ, et al. Monoclonal antibodies distinguish between wild and vaccine strains of yellow fever virus by neutralization, hemagglutination inhibition and immune precipitation of the virus envelope protein. Virology 125: 8-17, 1983. PubMed: 6187129
1015: Monath TP, et al. Yellow fever monoclonal antibodies: type-specific and cross-reactive determinants identified by immunofluorescence. Am. J. Trop. Med. Hyg. 33: 695-698, 1984. PubMed: 6206738
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