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UCD/PR 10.11
  • 平台编号:bio-68805
  • 规格:0.5ml
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生长状态:悬浮生长
ATCC Number:HB-8694?
是否是肿瘤细胞:0
物种来源:小鼠
运输方式:冻存运输
数量:大量
细胞形态:淋巴样
免疫类型:IgG
规格:0.5ml Designations: UCD/PR 10.11
Depositors: ?Regents of the University of California
Isotype: IgG
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against type 8 and type 18 cytokeratins
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with soluble extracellular cytokeratin.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts type 8 and type 18 cytokeratins in immunoblots.
The antibody can be used to detect cytokeratin in formalin or B-5 fixed paraffin sections.
The antibody primarily reacts with protein antigens in ductal portions of glands and with primary and metastatic adenocarcinomas.
Radioactively labeled UCD/PR 10.11 antibody can also be used to detect soluble extracellular cytokeratin in a quantitative sandwich radioimmunoassay.
Propagation: ATCC complete growth medium: Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products:recommended serum:ATCC 30-2020
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 3931: Cardiff RD, et al. Cytokeratin tumor markers and assays for their detection. US Patent 4,775,620 dated Oct 4 1988
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