欢迎访问中国微生物菌种查询网!   留言中心菌种专题|设为首页|加入收藏|
手机版
当前位置: 首页 > 细胞系 > ATCC普通细胞 > RA3-3A1/6.1
RA3-3A1/6.1
  • 平台编号:bio-68795
  • 规格:0.5ml
  • 拉丁属名:
  • 购买数量:
    订购
生长状态:悬浮生长
细胞形态:淋巴样
免疫类型:IgM
运输方式:冻存运输
是否是肿瘤细胞:0
物种来源:大鼠
ATCC Number:TIB-146?
数量:大量
规格:0.5ml Designations: RA3-3A1/6.1
Depositors: ?RL Coffman
Isotype: IgM
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology:lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against the B220 mouse B cell antigen (200000 dalton form of the T200 glycoprotein, CD45R)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with the mouse cell line RAW 112.
Spleen cells were fused with the S194/5.XX0.BU.1 myeloma.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 x 10 exp4 cells/ml, and do not allow the cell concentration to exceed 5 x 10 exp5 cells/ml.
References: 925: Coffman RL, Weissman IL. A monoclonal antibody that recognizes B cells and B cell precursors in mice. J. Exp. Med. 153: 269-279, 1981. PubMed: 6787164
927: Coffman RL, Weissman IL. B220: a B cell-specific member of the T200 glycoprotein family. Nature 289: 681-683, 1981. PubMed: 6970340
32948: Wong P, Rudensky AY. Phenotype and function of CD4+ T cells in mice lacking invariant chain. J. Immunol. 156: 2133-2142, 1996. PubMed: 8690902
上一篇:盐单胞菌属 下一篇:20.3 [TAb 250]

用户中心

联系方式
    官方网址: www.biobw.org
    联系电话:010-51291224
    官方邮箱:biobw11@sina.com
    联系地址:北京市丰台区造甲街110号
    咨询QQ