欢迎访问中国微生物菌种查询网!   留言中心菌种专题|设为首页|加入收藏|
手机版
当前位置: 首页 > 细胞系 > ATCC普通细胞 > WBE
WBE
  • 平台编号:bio-68510
  • 规格:0.2ml
  • 拉丁属名:
  • 购买数量:
    订购
年限:24 hours post fertilization embryo
运输方式:冻存运输
器官来源:胚胎
细胞类型:上皮细胞
细胞形态:上皮样
是否是肿瘤细胞:0
物种来源:其他
数量:大量
ATCC Number:CRL-2773?
生长状态:贴壁生长
规格:0.2ml Designations: WBE
Depositors: ?M Westerman
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Morone chrysops
Morphology:epithelial


Source: Organ: embryo
Cell Type: epithelial
Cellular Products:keratin [90251 ]
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: San Diego California,
Isolation date: February 22, 2001
Cytogenetic Analysis:Aneuploid. Range = 44 to 49. The chromosme morphology was normal for bass with all telocentric and acrocentric chromosomes. No dicentric or ring chromosomes were observed. [90251 ]
Age: 24 hours post fertilization embryo
Gender: unknown
Comments:These cells are useful for the study of host-pathogen interactions.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 31.0°C
Subculturing: Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 31?C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 31?C.

Subcultivation Ratio: 1:4 to 1:6 weekly
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 90251: Shimizu C, et al. Characterization of a white bass (Morone chrysops) embryonic cell line with epithelial features. In Vitro Cell. Dev. Biol. Anim. 39: 29-35, 2003. PubMed: 12892524
上一篇:盐单胞菌属 下一篇:WT 9-12

用户中心

联系方式
    官方网址: www.biobw.org
    联系电话:010-51291224
    官方邮箱:biobw11@sina.com
    联系地址:北京市丰台区造甲街110号
    咨询QQ