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FAT 7
  • 平台编号:bio-68326
  • 规格:48T
  • 拉丁属名:
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运输方式:冻存运输
年限:adult
ATCC Number:CRL-2109?
数量:大量
相关疾病:鳞状细胞癌
组织来源:nasal
生长状态:贴壁生长
细胞形态:上皮样
是否是肿瘤细胞:0
物种来源:大鼠
规格:48T Designations: FAT 7
Depositors: ?E Bermudez
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology:epithelial


Source: Tissue: nasal
Disease: squamous cell carcinoma
Cellular Products:transforming growth factor alpha (TGF alpha)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors:epidermal growth factor (EGF)
Tumorigenic:Yes
Age: adult
Gender: male
Comments:The FAT 7 line was established from a nasal squamous cell carcinoma induced by formaldehyde inhalation in an adult male rat.
The cells contain a point mutation in the conserved V region of the p53 gene at codon 271 (CGT --> CAT transversion).
Propagation: ATCC complete growth medium: Ham's F12K medium with 0.01 mg/ml insulin, 250 ng/ml hydrocortisone and 0.0025 mg/ml transferrin, 90%; fetal bovine serum, 10%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended
Medium Renewal: Twice per week
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin.
Add 1 to 2 ml of additional trypsin solution and let the culture sit at room temperature (or at 37C) until the cells detach.
Add fresh medium, aspirate and dispense into new flasks.
Subculture every 6 to 8 days.
Preservation: Culture medium, 95%; DMSO, 5%
Doubling Time: 24 hrs
References: 22639: Bermudez E, et al. Characterization of cell lines derived from formaldehyde-induced nasal tumors in rats. Mol. Carcinog. 9: 193-199, 1994. PubMed: 8148052
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