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HS-5
  • 平台编号:bio-68141
  • 规格:0.1ml
  • 拉丁属名:
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ATCC Number:CRL-11882?
运输方式:冻存运输
细胞形态:成纤维样
数量:大量
年限:30 years
器官来源:骨髓
生长状态:贴壁生长
组织来源:stroma
细胞类型:其他细胞类型
是否是肿瘤细胞:0
物种来源:人
规格:0.1ml Designations: HS-5
Depositors: ?Fred Hutchinson Cancer Res. Cntr.
Biosafety Level:2 [Cells contain human papilloma viral sequences ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens deposited as human
Morphology:fibroblast


Source: Organ: bone marrow
Tissue: stroma
Cell Type: HPV-16 E6/E7 transformed
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:HS-23 and HS-27A secrete low levels of growth factors and do not support proliferation of isolated progenitor cells in cocultures.
The cell line can also be used as a feeder layer in ex vivo bone marrow cultures or in colony forming assays.
Age: 30 years
Gender: male
Ethnicity: Caucasian
Comments:Cellular Products: : granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage-CSF (GM-CSF), macrophage-CSF (M-CSF), Kit ligand (KL), macrophage-inhibitory protein-1 alpha, interleukin-1 alpha (IL-1alpha), IL-1beta, IL-1RA, IL-6, IL-8, IL-11, and leukemia inhibitory factor (LIF)
Long term bone marrow cells were transformed with the amphotropic retrovirus vector LXSN16E6E7 in the presence of polybrene.
Twenty-seven immortalized clones designed HS-1 to HS-27 were isolated.
One of these cell lines HS-27A (CRL-2496) has been deposited in the ATCC 's general collection. One cell line (HS-5) has been deposited in the Patent Depository (CRL-11882).
HS-5 supports proliferation of hematopoietic progenitor cells when cocultured in serum-deprived media with no exogenous factors.
HS-23 and HS-27A secrete low levels of growth factors and do not support proliferation of isolated progenitor cells in cocultures.
The cell line can also be used as a feeder layer in ex vivo bone marrow cultures or in colony forming assays.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37?C.

      Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:9 is recommended
      Medium Renewal: Everh 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 40180: Roecklein BA, Torok-Storb B. Functionally distinct human marrow stromal cell lines immortalized by transduction with the human papilloma virus E6/E7 genes. Blood 85: 997-1005, 1995. PubMed: 7849321
44213: Torok-Storb B, et al. Human marrow stromal cell lines which sustain hematopoieses. US Patent 5,879,940 dated Mar 9 1999
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