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HEPM
  • 平台编号:bio-68112
  • 规格:48T
  • 拉丁属名:
  • 购买数量:
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年限:fetus
是否是肿瘤细胞:0
物种来源:人
生长状态:贴壁生长
细胞形态:成纤维样
ATCC Number:CRL-1486?
运输方式:冻存运输
组织来源:palatal mesenchyme
数量:大量
规格:48T Designations: HEPM
Depositors: ?M Macy
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast


Source: Tissue: palatal mesenchyme
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: July, 1979
Applications:transfection host
Receptors:epidermal growth factor (EGF)
DNA Profile (STR):Amelogenin: X
CSF1PO: 10,11
D13S317: 8,12
D16S539: 11,12
D5S818: 11,13
D7S820: 8,10
THO1: 6,9.3
TPOX: 8,11
vWA: 17,18
Cytogenetic Analysis:This is a human diploid cell line with the 46,XX karyotype. The modal chromosome number was 46, occurring in 76% of cells. The rate of cells with polyploidies was 4.2%. No consistent chromosome aberrations were detected.
Age: fetus
Gender: female
Comments:The cells are highly responsive to epidermal growth factor (EGF).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37?C.

      Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
      Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 1067: Yoneda T, Pratt RM. Mesenchymal cells from the human embryonic palate are highly responsive to epidermal growth factor. Science 213: 563-565, 1981. PubMed: 7017936
1068: Yoneda T, Pratt RM. Interaction between glucocorticoids and epidermal growth factor in vitro in the growth of palatal mesenchymal cells from the human embryo. Differentiation 19: 194-198, 1981. PubMed: 6458523
58036: Yoneda T, Pratt RM. Glucocorticoid receptors in palatal mesenchymal cells from the human embryo: relevance to human cleft palate formation. J. Craniofacial Genet. Dev. Biol. 1: 411-423, 1981.
58037: Pratt RM, et al. Prescreening for environmental teratogens using cultured mesenchymal cells from the human embryonic palate. Teratog. Carcinog. Mutagen. 2: 313-318, 1982. PubMed: 6130630
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