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Hs68
  • 平台编号:bio-68110
  • 规格:0.1ml
  • 拉丁属名:
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数量:大量
细胞类型:成纤维细胞
运输方式:冻存运输
器官来源:皮肤
是否是肿瘤细胞:0
物种来源:人
生长状态:贴壁生长
细胞形态:成纤维样
ATCC Number:CRL-1635?
相关疾病:其他疾病
年限:newborn
规格:0.1ml Designations: Hs68
Depositors: ?RB Owens
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast


Source: Organ: skin; foreskin
Disease: aspartoacylase deficiency; possible Canavan disease
Cell Type: fibroblast
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 9,12
D13S317: 11,13
D16S539: 11,12
D5S818: 8,11
D7S820: 11,12
THO1: 6,9.3
TPOX: 11
vWA: 16
Cytogenetic Analysis:This is a human cell line with the normal 46,XY karyotype. The modal chromosome number was 46, with polyploidy at 3.6%. Both X and Y chromosomes can be identified clearly.
Age: newborn
Gender: male
Ethnicity: Caucasian
Comments:Hs68 is one of a series of human foreskin fibroblast lines developed at the Naval Biosciences Laboratory (NBL) in Oakland, CA. The material was obtained from an apparently normal Caucasian newborn male in February, 1969.
Longevity studies carried out at NBL demonstrated that the cells could be propagated for 42 passages.
The ATCC has been informed by Dr. Anne B. Johnson, Department of Pathology and Neuroscience at Albert Einstein College of Medicine, that this cell line has an unusually low level of the enzyme aspartoacylase (0.028 mU/mg or less on repeat assays.
Our records indicate that the donor of Hs 68 was apparently normal, but Dr. Johnson pointed out that individuals exhibiting a deficiency in aspartoacylase suffer from Canavan disease.
Canavan disease is a devastating, autosomal recessive disorder involving spongy degeneration of the nervous system.
According to Dr. Johnson, fibroblasts from affected children have aspartoacylase levels of 0.003 to 0.02 mU/mg; carriers' levels vary from 0.016 to 0.04 mU/mg and normals run from 0.1 to 0.4 mU/mg.
More information on aspartoacylase and Canavan disease can be found in an article by R. Kaul et al. [26119 ]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 26119: Kapatos G. Tetrahydrobiopterin synthesis rate and turnover time in neuronal cultures from embryonic rat mesencephalon and hypothalamus. J. Neurochem. 55: 129-136, 1990. PubMed: 2355214
33098: Sayles PC, Johnson LL. Intact immune defenses are required for mice to resist the ts-4 vaccine strain of Toxoplasma gondii. Infect. Immun. 64: 3088-3092, 1996. PubMed: 8757838
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