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ATCC -VRMC-11™
  • 平台编号:bio-49809
  • 规格:frozen
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ATCC® Number:VRMC-11™
Classification: Polyomaviridae, Polyomavirus
Agent: Simian virus 40
Strain: pUCSVH388-16 [molecular clone of Simian virus 40, SV-40]
Depositors: JA Lednicky, JS Butel
Biosafety Level:2
Shipped: frozen
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Incubation : Temperature: 37.0°C
Comments : Description of cloned agent: Name: SV40 Strain: H388, variant #16 Classification: Papovavirus Contains a full-length non-archetypal SV40-H388 genome cloned into pUC-19 at EcoRI site; isolated from peripheral blood mononuclear cell DNA of animal H388 by J. Lednicky. [111558] Plasmid size: 7893 bp [pUC-19 (vector): 2686 bp + SV40-H388 (insert): 5207 bp]. High-copy number; pUC-19 has a ColEI ori. [111558] VRMC-11 (variant #16) is one of a matched set of 3 clones of regulatory region variants (see also VRMC-9 and VRMC-10). Plasmid might dimerize; restriction pattern will not change. Restriction enzyme analysis: BamHI--4.479,3.414; EcoRI--5.207,2.686; KpnI--6.392,1.501; SalI--7.893; SfiI--7.893; XhoI--uncut. [111558] Sequence (viral regulatory region, T-ag carboxy terminus, and part of VP1 gene) determined by: Xian Min Dai. Clone viability: not tested. [111558]
References : 36787: Lednicky JA, et al. Natural isolates of simian virus 40 from immunocompromised monkeys display extensive genetic heterogeneity: new implications for polyomavirus disease. J. Virol. 72: 3980-3990, 1998. PubMed: 9557685 111558: John A Lednicky, personal communication
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