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ATCC -30335™
  • 平台编号:bio-48471
  • 规格:frozen
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ATCC® Number:30335™
Organism: Crypthecodinium cohnii (Seligo) Javornicky
Designations: MC 5
Isolation: Phosphorescent Bay, Puerto Rico, 1957 (?)
Depositors: CA Beam
History: ATCC<<--CA Beam<<--J.J.A. McLaughlin
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 460: A2E6 mediumTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 30021 SPEC: This culture is routinely shipped as a frozen stabilate. Thaw the ampule and aseptically transfer the material to 5 ml of ATCCmedium 460 in a 16 x 125 mm screw-capped test tube. Do not distribute the thawed material to a larger volume of medium. It is essential to first establish the culture in a small volume. Screw cap on tightly, loosen one half turn, and incubate culture upright at 25C. The culture should be ready to subculture in approximately 7 days. To subculture, screw the cap on tightly, invert the culture 5 times and aseptically transfer a 0.1 ml aliquot to 5 ml of ATCCmedium 460 and incubate as above. Prepare two subcultures weekly. Retain all cultures for up to one month to ensure against loss.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Minor Sibling Species MC Sexual isolation and genetic diversification [4886] sexuality and meiosis [21756] cryopreservation [23828] use of plastic ampoules for freeze preservation [23870]
Classification: KINGDOM: Protozoa
References: 4783: Salt GW. Changes in the cell volume of Didinium nasutum during population increase. J. Protozool. 22: 112-115, 1975. 4790: Beam CA, Himes M. Sexual isolation and genetic diversification among some strains of Crypthecodinium cohnii-like dinoflagellates evidence of speciation. J. Protozool. 24: 532-539, 1977. 4886: Provasoli L, Gold K. Nutrition of the American strain of Gyrodinium cohnii. Arch. Mikrobiol. 42: 196-203, 1962. PubMed: 14489006 21756: Beam CA, Himes MSexuality and meiosis in dinoflagellatesIn: Beam CA, Himes MBiochemistry and physiology of protozoa, 2nd ed.3New YorkAcademic Press171-206, 1980 23828: Simione FP Jr., Daggett PM. Recovery of a marine dinoflagellate following controlled and uncontrolled freezing. Cryobiology 14: 362-366, 1977. PubMed: 560941 23870: Simione FP Jr., et al. The use of plastic ampoules for freeze preservation of microorganisms. Cryobiology 14: 500-502, 1977. PubMed: 891238
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