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ATCC -50424™
  • 平台编号:bio-47541
  • 规格:dried
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ATCC® Number:50424™
Organism: Adriamonas sp. 1
Designations: MD-6
Depositors: CJ O'Kelly
History: ATCC<<--CJ O'Kelly<<--T.A. Nerad
Biosafety Level:1
Shipped: dried
Growth Conditions: ATCCmedium 802: Sonneborn's Paramecium mediumTemperature: 25.0°C Protocol: ATCCNO: 50397 SPEC: Store vial in refrigerator until ready to open it. Open sealed outer glass vial according to instructions provided with the vial. Rehydrate the material by adding 0.5 ml of ATCCmedium 802 bacterized with Enterobacter aerogenes ATCC13048. (Bacterize the medium by inoculating it with a loopful of bacteria from an agar slant at least 24 hours prior to inoculatiion with the flagellate.) Aseptically remove the shredded filter paper pellet with sterile forceps and add a T-25 tissue flask containing 10 ml of bacterized medium. Aseptically remove the remainder of the fluid from the glass vial and add it to the T-25 flask. Screw the cap on tightly and incubate the flask at 25C. Transfer every 21-28 days by vigorously agitating the flask and aseptically transferring 0.1 ml to a fresh flask of bacterized medium.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Classification: KINGDOM: Protozoa
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