ATCC Medium: 1246 Mineral Medium for Hydrogen Bacteria

中国微生物菌种查询网 / 2018-01-15 13:53:17

ATCC Medium: 1246 Mineral Medium for Hydrogen Bacteria

Basal Medium

KH2PO4 ......................................................2.3 g
Na2HPO4 x 2H2O........................................2.9 g
NH4Cl .........................................................1.0 g
MgSO4 x 7H2O...........................................0.5 g
NaHCO3 .....................................................0.5 g
CaCl2 x 2H2O………………........................0.01 g
DI Water…………………………..................980.0 ml
Agar (if needed)..........................................15.0 g
Autoclave at 121?C. After basal medium has cooled aseptically add the following
solution:
Ferric Ammonium Citrate Solution
Ferric ammonium citrate..............................0.05 g
DI Water………………………………………20 ml
Filter sterilize.
Incubate at 30?C under a gas mixture of 60% H2, 10% CO2, 25% N2, 5% O2

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