ATCC medium: 484 PPLO Broth w/ Additives for Mycoplasma
PPLO broth w/o CV (BD 255420)........................14.7 g
Arginine HCl.........................................................2.1 g
Agar, Noble (BD 214230) (if needed)..................10.0 g
DI Water…………………………...........................700.0 ml
Autoclave at 121C for 15 minutes.
Cool to 50C and aseptically add the following filter-sterilized components:
Glutamine.............................................................1.46 g
Phenol red............................................................20.0 mg
100X MEM Vitamins (GIBCO 11120-052)............1.0 ml
Horse serum.........................................................200.0 ml
Yeast Extract Solution (GIBCO 18180)................100.0 ml
Adjust medium, if necessary, for final pH 7.1.