ATCC Medium: 2847 GYP w/ salts
Base Medium
Yeast Extract……………………………10 g
Peptone…………………………………10 g
Sodium acetate…………………………10 g
Salt B Solution (see below)……………5 ml
DI Water………………………………895 ml
Adjust pH to 6.8. Autoclave at 121C. When medium is cooled, aseptically add the
following filter sterilized solution:
Glucose Solution
Glucose………………………………….20 g
DI Water………………………………….100 ml
Salt B Solution
MgSO4 x 7 H2O……………………….4 g
FeSO4 x 7H2O………………………..0.2 g
NaCl……………………………………0.2 g
MnSO4 x 4H2O……………………….0.16 g
DI Water……………………………….100 ml