ATCC medium: 1842 Sulfurospirillum medium
Solution A:
KH2PO4 .........................................1.36 g
MgSO4 . 7H2O ...................................0.37 g
CaCl2 . 2H2O ...................................0.10 g
NH4Cl ..........................................0.27 g
Distilled water..............................953.0 ml
Solution B:
NaHCO3 .........................................2.0 g
Trace Elements Solution SL-10 (see below)......2.0 ml
Sodium fumarate................................4.0 g
Distilled water...............................40.0 ml
Filter-sterilize.
Solution C:
L-Cysteine .
HCl...............................0.063 g
Distilled water................................5.0 ml
Prepare fresh and filter-sterilize. Autoclave Solution A at 121C for 15 minutes.
Cool under an atmosphere of 90% N2, 10% CO2. Aseptically add Solutions B and C.
Adjust medium, if necessary, to pH 7.2 with sterile solutions of 2 M Na2CO3 or 2 N HCl.
Trace Elements
Solution SL-10:
HCl (25%).........................10.0 ml
FeCl2 . 4H2O .......................1.5 g
ZnCl2 .............................70.0 mg
MnCl2 . 4H2O .....................100.0 mg
H3BO3 ..............................6.0 mg
CoCl2 . 6H2O .....................190.0 mg
CuCl2 . 2H2O .......................2.0 mg
NiCl2 . 6H2O ......................24.0 mg
Na2MoO4 . 2H2O.....................36.0 mg
Distilled water..................990.0 ml
Dissolve FeCl2 in the HCl, dilute with water, add and dissolve the other salts, adjust pH to 6.0 with NaOH, and fill up to 1.0 L.