ATCC medium: 2216 V8 Juice Agar, Quarter-strength
V8 Juice..........................50.0 ml
CaCO3 ..............................0.75 g
Agar..............................15.0 g
Distilled deionized water........950.0 ml
Add tap water to 1000 ml. Adjust to pH 7.2 +/- 0.2 Autoclave at 121C for 15 minutes.