ATCC Medium: 11 Azotobacter Supplement (Glucose/pH 7.6)
K2HPO4…………………………………..1.0 g
MgSO4 x 7H20…………………………...0.2 g
NaCl………………………………………..0.2 g
FeSO4……………………………….…….5.0 mg
Soil Extract (See Below)…………..…….100 ml
Glucose Solution (See Below)….………100 ml
Agar……………………………….……….15.0 g
DI water…………………………….……...800 ml
Adjust base medium to pH 7.6.
Autoclave at 121oC.
Glucose Solution
Glucose………………………20 g
DI Water……………………...100 ml
Filter sterilize and add to
sterile base. Soil
Extract African Violet Soil……………77.0 g
Na2CO3………………………0.2 g
DI water………………………200 ml
Boil for 1 hour. Filter to clarify before use.