ATCC medium: 1101 Sphaericus spore medium
Nutrient agar...............23.0 g
Yeast extract................0.5 g
MnCl2 ........................6.0 mg
CaCl2 .......................78.0 mg
MgCl2 .......................95.0 mg
Distilled water..............1.0 L
Autoclave at 121C for 15 minutes.