ATCC medium: 55 Nutrient agar with dihydrostreptomycin 625 mcg/ml
Prepare Nutrient Agar (BD 213000) per manufacturer's instructions.
Autoclave at 121C for 15 minutes. Prepare a solution of dihydrostreptomycin, filter-sterilize, and add sufficient amount to achieve a final concentration in the medium of 625 mcg/ml.