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观叶植物灰霉病毒

观叶植物灰霉病毒

  • 平台编号:bio-110617
  • 规格:试纸条(5 次测试/25 次测试)
  • 注意事项:仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用
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Catalog Number AS 049 OR 25 Intended Use The EnviroLogix QuickStix Kit for Botrytis in Ornamentals screens for the presence of Botrytis cinerea, often called gray mold or botrytis blight in ornamentals. The strip can detect the presence of Botrytis cinerea at an early stage, when it is difficult to differentiate symptoms from other bacterial, viral or fungal infections and/or insect damage. How the Test Works This test is for use on leaf or petal tissue that is suspected to be infected with Botrytis. To detect infected tissue with the EnviroLogix QuickStix Strip, samples must be extracted with the buffer provided. Each QuickStix Strip has an absorbent pad at both ends. The protective tape with the arrow indicates which end of the strip to insert into the extraction tube. The sample travels up the membrane strip and is absorbed into the larger pad at the top of the strip. The portion of the strip between the protective tape and the absorbent pad at the top of the strip is used to view the reactions as described under “Interpreting the Results”. Sample Preparation Sampling Requirement: Take a petal or leaf sample that includes a contiguous suspect spot or area of 4-5 mm in size. At least one of the two punch samples (Step 1) must include this size of a spot/area to ensure accurate detection. The punch cap results in a sample of approximately 10 mm diameter. Important: Allow buffer to come to room temperature before using. 1. Sandwich a section of petal or leaf tissue between the cap and body of the Disposable Tissue Extractor tube. Snap two circular punches by closing the cap. Push the sample down into the tapered bottom of the tube with the pestle and grind for 30 seconds. Write the sample identification on the tube with a waterproof marker. 2. Holding the dropper bottle vertically, add 12 drops of buffer to the tissue sample. Re-grind the tissue by rotating the pestle against the sides of the tube with twisting motions. Continue this process for 20 to 30 seconds, or until the leaf tissue is well ground. Remove and discard pestle. How to Run the QuickStix Strip Test 1. Allow refrigerated canisters to come to room temperature before opening. Remove the QuickStix Strips to be used. Avoid bending the strips. Reseal the canister immediately. 2. Place the strip into the extraction tube. The sample will travel up the strip. Use a rack to support multiple tubes if needed. 3. Allow the strip to develop for a full 10 minutes before making final assay interpretations. Positive sample results may appear much more quickly. Read and interpret the results as close as possible to the ten-minute time point, while the strip is still in the extraction tube. 4. To retain the strip, cut off and discard the bottom section of the strip covered by the arrow tape. QuickStix Kit for Botrytis in Ornamentals Page 2 of 4 Rev. 02-10-10 Add a test strip, wait 10 minutes Any pink test line is considered positive Interpreting the Results Development of the Control Line within 10 minutes indicates that the strip has functioned properly. Any strip that does not develop a Control Line should be discarded and the sample re-tested using another strip. If the sample extract contains Botrytis, a second line (Test Line) will develop on the membrane strip between the Control Line and the protective arrow tape. The results should be interpreted as positive for Botrytis. If no Test Line is observed at 10 minutes, the results should be interpreted as negative. Kit Storage This QuickTox Kit should be stored refrigerated. Note the shelf life on the kit box. The kit may be used in field applications; however, prolonged exposure to high temperatures may adversely affect the test results. Do not open the desiccated canister until ready to use the strips. Cross Reactivity The antibody used to produce this kit was found to be reactive with: The antibody used to produce this kit was found to be not reactive with: • Botrytis cinerea (various isolates) • B. byssoidea • B. allii (various isolates) • B. fabae • B. squamosa • B. stokesii (tulipae; various isolates) • B. streptothrix • B. tulipae • B. aclada • B. elliptica • Monilinia fructicola • M. laxa • M. lambertella • Sclerotinia spp. • Alternaria infectoria • Alternaria alternata • Aspergillus niger • Aureobasidium pullulans • Cladosporium macrocarpum • Cladosporium herbarum • Cladosporium paeoniae • Coniella fragaria • M. hiemalis • Rhizopus spp. • Stemphyllium spp. • Trichoderma harzianum • T. viride • Ulocladium spp. Precautions and Notes • This kit is designed for screening the presence or absence of Botrytis and is not meant to be quantitative. • Important Note: The kit will detect its target pathogen regardless of the pathogen’s viability. It should not be used to determine the efficacy of treatment efforts, because although the pathogen may be rendered non-viable, the protein is still present and will cause a positive result. • As with all tests, it is recommended that results be confirmed by an alternate method. • The assay has been optimized to be used with the protocol provided in the kit. Deviation from this protocol may invalidate the results of the test. Compositing or pooling of samples is not recommended and may result in false negative results. • A negative result does not preclude the presence of Botrytis infection in other areas or at other times. QuickStix Kit for Botrytis in Ornamentals Page 3 of 4 Rev. 02-10-10 • A strong positive result may safely be interpreted in as little as 5 minutes after sample addition. It is not safe, however, to conclude that a sample is negative before a full 10 minutes has elapsed. A weakly positive sample may require the full 10 minutes for a distinct Test Line to appear. • Protect all components from hot or cold extremes of temperature when not in use. Do not leave in direct sunlight or in vehicle. A small portable cooler is recommended for field testing applications to protect the kit from extreme temperatures. 
安瓿瓶冻干管打管说明
 
一、打管说明
1、安瓿瓶开封:用浸过75%酒精的脱脂棉擦净安瓿管,用火焰加热其顶端,滴少量无菌水至加热顶端使之破裂,用锉刀或者镊子敲下已破裂的安瓿管顶端。
2、菌株恢复培养:用无菌吸管吸取0.3--0.5ml适宜的液体培养基,滴入安瓿管内,轻轻振荡,使冻干菌体溶解呈悬浮状。吸取全部菌体悬浮液,移植于1-2支建议的培养基试管中,并在建议的条件下培养。
3、注意事项:菌种活化前,请将安瓿管保存在6-10℃的环境下,某些菌种经过冷冻干燥保存后,延迟期较长,需要连续两次继代培养才能正常生长。
4、复苏后的菌种在传1-2代后使用。
5、暂不启开的安瓿及复苏后需保藏的斜面应于4℃中保藏。
特别注意事项
l、微生物菌种应保藏于低温、清洁和干燥的地方,室温放置时问过长会导致菌种衰退;
2、菌种操作应在无菌条件下进行,防止杂菌污染:
3、斜面菌种保藏时间通常为1-2个月,应根据菌种状况及时转接;冻干菌种保藏时间通常为5~10年;
4、菌种使用过程中如出现杂菌污染或菌种生产性能下降,应及时与中国微生物菌种查询网联系或更换新的菌种。

  西林瓶和甘油冻存管打管说明
 
二、西林瓶菌种打管复活操作步骤
1、用 75%酒精棉擦拭西林瓶表面进行消毒。 
2、在生物安全柜中打开铝制瓶盖和胶盖。 
3、用无菌吸管吸取 0.5ml 左右液体培养基(《菌种说明书》中固体培养基去掉琼脂即可)于西 林瓶中将冻干菌粉全部溶解。 
4、将溶解后的菌悬液转移至盛有 4~5mL 液体培养基的试管中混匀,可将残留在吸管中的 1-2 滴菌悬液转接至固体培养基上。
5、将液体试管和斜面试管于推荐条件下静置培养,以液体培养结果为准。
三、甘油冻存管菌种打管复活操作步骤 
1、准备好推荐的菌种培养基(见《菌种说明书》)。
2、将冻存管下半部浸入 37℃温水中轻轻摇动,使冷冻状态的菌种悬液迅速融化。如果收到菌 种冻存管时菌悬液是融化状态则需要立即进行转接培养。 3、用 75%酒精棉擦拭冻存管表面进行消毒。
4、在生物安全柜中打开冻存管瓶盖,用无菌吸管吸取全部菌悬液,均匀涂布到 2 支试管斜面 或平板上。 
5、将斜面试管或平板于推荐条件下静置培养。对于生长缓慢的菌种应持续培养 2 周至更长的 时间。
四、注意事项 
1、 所有打管操作都需要无菌操作。需由专业微生物技术人员在相应防护设备中进行,生物危 害程度为三类的菌种应在生物安全柜中操作。 
2、 西林瓶和冻存管打开后需一次用完,不能留存。 
3、 菌种经过冻干保藏后,处于休眠状态,需要转接 2-3 代恢复活力。
4、 大部分冻干菌种会在培养几天后生长。有些菌种第一代恢复生长时会有较长的延迟期,培养此类菌种时需要将培养时间延长至正常培养时间的双倍时长,甚至 2 周至更长的时间。