Name:
Clostridioides difficile (Hall and O'Toole 1935) Lawson et al. 2016
DSM No.:
27147
Strain designation:
R20291
Other collection no.
or WDCM no.:
NCTC 13366
Isolated from:
Faeces from a symptomatic patient. Outbreak at Stoke Mandeville Hospital, UK
Country:
United Kingdom of Great Britain and Northern Ireland
Buckinghamshire, Aylesbury
Date of sampling:
before 22.04.2013
Nagoya Protocol Restrictions:
There are NO known Nagoya Protocol restrictions for this strain.
History:
<- P. Bracegirdle, Health Protection Agency Culture Collections, Salisbury, United Kingdom; NCTC 13366
Genbank accession numbers:
complete genome: FN545816
Cultivation conditions:
Medium 78 , anaerobic, 37°C
or
Medium 1203 , anaerobic, 37°C
Incubation time: 1-2 days
Please follow special instructions: 'Cultivation of Anaerobes'
Complete DSMZ Media List
Summary and
additional information:
<- P. Bracegirdle, Health Protection Agency Culture Collections, Salisbury, United Kingdom; NCTC 13366. Faeces from a symptomatic patient. Outbreak at Stoke Mandeville Hospital, UK; United Kingdom, Buckinghamshire, Aylesbury. Taxonomy/description (26004, 26018). Sequence accession no. complete genome: FN545816. Ribotype 027 with 18bp deletion in tcdC gene. Genome sequenced. Positive PCR for toxins A and B (tcdA and tcdB) and the binary toxin (ctdA and ctdB).. (Medium 78, 37°C, anaerobic or Medium 1203, 37°C, anaerobic).
Literature:
26004, 26018
Risk group:
2 (classification according to German TRBA)
Restrictions:
Act dealing with the prevention & control of infectious diseases in man (Infektionsschutzgesetz), Category A1
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about Clostridioides difficile DSM 27147