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Bordetella bronchiseptica

Bordetella bronchiseptica

  • 平台编号:bio-109749
  • 拉丁属名: Bordetella bronchiseptica
  • 注意事项:仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用
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Bacteria Collection: Bordetella bronchiseptica
NCTC Number: NCTC 8344
Current Name: Bordetella bronchiseptica
Original Strain Reference: NRRL B-140
Other Collection No: ATCC 4617; DSM 10303; NCIB 9935; NRRL B-140; PCI 1701; WHO 11
Previous Catalogue Name: Bordetella bronchiseptica
Type Strain: No
Family: Alcaligenaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: nutrient agar,37, aerobic
Conditions for growth on liquid media: nutrient broth,37, aerobic
16S rRNA Gene Sequence: >gb|AJ278452|DSM 10303|Bordetella bronchiseptica partial 16S rRNA gene, strain DSM 10303.| agagtttgatcatgg...
Bibliography: 1973 BRITISH PHARMACOEPIA 104
Extended Bibliography: showhide Show bibliography
Ref #: 14541
Author(s): von Wintzingerode,F.;Schattke,A.;Siddiqui,R.A.;Rosick,U.;Gobel,U.B.;Gross,R.
Journal: Int J Syst Evol Microbiol
Title: Bordetella petrii sp. nov., isolated from an anaerobic bioreactor, and emended description of the genus Bordetella
Volume: 51
Page(s): 1257-65
Year: 2001
Keyword(s): GENBANK/AJ242553 GENBANK/AJ242599 Anaerobiosis Base Composition Base Sequence Bioreactors Bordetella/*classification/genetics/*isolation & purification/metabolism Chlorobenzenes/metabolism DNA, Bacterial/chemistry/genetics DNA, Ribosomal/genetics Genes, Bacterial Geologic Sediments/microbiology Microscopy, Electron Molecular Sequence Data Open Reading Frames Phylogeny Selenium Compounds/metabolism Terminology
Remarks: A novel Bordetella species was isolated from an anaerobic, dechlorinating bioreactor culture enriched from river sediment. The only strain, Se-1111R(T) (= DSM 12804T = CCUG 43448T), for which the name Bordetella petrii is proposed, is designated the type strain of the novel species. Strain Se-1111R(T) was isolated from the dechlorinating mixed culture due to its ability to anaerobically reduce selenate to elemental selenium. Comparative 16S rDNA sequence analysis showed a close relationship between Se-1111R(T) and members of the genus Bordetella within the beta-Proteobacteria. This close phylogenetic relatedness was also reflected in several metabolic properties of Se-1111R(T), including its incapacity to utilize carbohydrates, by the high G+C content (63.8 mol%) of its DNA and by the presence of Q-8 as the major isoprenoid quinone. DNA-DNA hybridization experiments with type strains of all species of the genus Bordetella and closely related species Achromobacter xylosoxidans subsp. denitrificans provided further evidence for the assignment of strain Se-1111R(T) as a novel species of the genus Bordetella. This genus currently consists of seven aerobic species, all of which are known to occur in close pathogenic, opportunistic or possibly commensal relationships with various host organisms. B. petrii is the first member of this genus isolated from the environment and capable of anaerobic growth. The proposal of the novel species and an emended description of the genus Bordetella is presented.
URL: 11491321
Ref #: 12260
Author(s): von Wintzingerode,F.;Schattke,A.;Siddiqui,R.A.;Rosick,U.;Gobel,U.B.;Gross,R.
Journal: Int J Syst Evol Microbiol
Title: Bordetella petrii sp. nov., isolated from an anaerobic bioreactor, and emended description of the genus Bordetella
Volume: 51
Page(s): 1257-65
Year: 2001
Keyword(s): GENBANK/AJ242553 GENBANK/AJ242599 Anaerobiosis Base Composition Base Sequence Bioreactors Bordetella/*classification/genetics/*isolation & purification/metabolism Chlorobenzenes/metabolism DNA, Bacterial/chemistry/genetics DNA, Ribosomal/genetics Genes, Bacterial Geologic Sediments/microbiology Microscopy, Electron Molecular Sequence Data Open Reading Frames Phylogeny Selenium Compounds/metabolism Support, Non-U.S. Gov't Terminology
Remarks: A novel Bordetella species was isolated from an anaerobic, dechlorinating bioreactor culture enriched from river sediment. The only strain, Se-1111R(T) (= DSM 12804T = CCUG 43448T), for which the name Bordetella petrii is proposed, is designated the type strain of the novel species. Strain Se-1111R(T) was isolated from the dechlorinating mixed culture due to its ability to anaerobically reduce selenate to elemental selenium. Comparative 16S rDNA sequence analysis showed a close relationship between Se-1111R(T) and members of the genus Bordetella within the beta-Proteobacteria. This close phylogenetic relatedness was also reflected in several metabolic properties of Se-1111R(T), including its incapacity to utilize carbohydrates, by the high G+C content (63.8 mol%) of its DNA and by the presence of Q-8 as the major isoprenoid quinone. DNA-DNA hybridization experiments with type strains of all species of the genus Bordetella and closely related species Achromobacter xylosoxidans subsp. denitrificans provided further evidence for the assignment of strain Se-1111R(T) as a novel species of the genus Bordetella. This genus currently consists of seven aerobic species, all of which are known to occur in close pathogenic, opportunistic or possibly commensal relationships with various host organisms. B. petrii is the first member of this genus isolated from the environment and capable of anaerobic growth. The proposal of the novel species and an emended description of the genus Bordetella is presented.
URL: 21383404
Ref #: 1376
Author(s): Kirshbaum,A.;Arret,B.
Journal: Antibiot. Chemother.
Title: Outline of details for assaying the commonly used antibiotics.
Volume: 9
Page(s): 613-617
Year: 1959
Ref #: 175
Author(s): Simon,H.J.;Yin,E.J.
Journal: Appl. Microbiol.
Title: Microbioassay of antimicrobial agents.
Volume: 19
Page(s): 573-597
Year: 1970
Ref #: 4759
Journal: U. S. Pharmacopeia 22nd rev.
Page(s): 1488-1493
Year: 1990
Ref #: 6152
Author(s): Simon,H.J.;Yin,E.Y.
Journal: Appl. Microbiol.
Title: Microbioassay of antimicrobial agents.
Volume: 19
Page(s): 573-579
Year: 1970
Ref #: 6155
Author(s): Kavanagh,F.
Journal: Antibiot. Chemother.
Volume: 7
Page(s): 639-640
Year: 1957
Ref #: 6263
Author(s): Kavanagh,F.(Ed.)
Title: Analytical microbiology
Page(s): 349-352
Year: 1963
Data: (ATCC 4617, PCI 1701, WHO 11, NCIB 9935) / ATCC in 1951 / Assay of Polymyxin B, colistin, sulphomethate, sulphomyxin and Dimethylchlortetracycline / British Pharmacopoeia 1973, p. 104
Accession Date: 01/01/1951
Authority: (Ferry 1912) Moreno-Lopez 1952 (AL)
Depositor: ATCC
Taxonomy: TaxLink: S579 (Bordetella bronchiseptica (Ferry 1912) Moreno-Lopez 1952) - Date of change: 5/02/2003
Other: ANTIBIOTIC (AND OTHER ANTIMICROBIALS) ASSAY, SENSITIVITY, RESISTANCE CONTROL STRAINSColistin...
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country
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Note:
The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

Cultures supplied by Culture Collections are to be used as controls for microbiology testing and for research purposes only. Please view the Terms & Conditions of Supply for more information.

Contact us if you want to discuss commercial use of the cultures.

安瓿瓶冻干管打管说明
 
一、打管说明
1、安瓿瓶开封:用浸过75%酒精的脱脂棉擦净安瓿管,用火焰加热其顶端,滴少量无菌水至加热顶端使之破裂,用锉刀或者镊子敲下已破裂的安瓿管顶端。
2、菌株恢复培养:用无菌吸管吸取0.3--0.5ml适宜的液体培养基,滴入安瓿管内,轻轻振荡,使冻干菌体溶解呈悬浮状。吸取全部菌体悬浮液,移植于1-2支建议的培养基试管中,并在建议的条件下培养。
3、注意事项:菌种活化前,请将安瓿管保存在6-10℃的环境下,某些菌种经过冷冻干燥保存后,延迟期较长,需要连续两次继代培养才能正常生长。
4、复苏后的菌种在传1-2代后使用。
5、暂不启开的安瓿及复苏后需保藏的斜面应于4℃中保藏。
特别注意事项
l、微生物菌种应保藏于低温、清洁和干燥的地方,室温放置时问过长会导致菌种衰退;
2、菌种操作应在无菌条件下进行,防止杂菌污染:
3、斜面菌种保藏时间通常为1-2个月,应根据菌种状况及时转接;冻干菌种保藏时间通常为5~10年;
4、菌种使用过程中如出现杂菌污染或菌种生产性能下降,应及时与中国微生物菌种查询网联系或更换新的菌种。

  西林瓶和甘油冻存管打管说明
 
二、西林瓶菌种打管复活操作步骤
1、用 75%酒精棉擦拭西林瓶表面进行消毒。 
2、在生物安全柜中打开铝制瓶盖和胶盖。 
3、用无菌吸管吸取 0.5ml 左右液体培养基(《菌种说明书》中固体培养基去掉琼脂即可)于西 林瓶中将冻干菌粉全部溶解。 
4、将溶解后的菌悬液转移至盛有 4~5mL 液体培养基的试管中混匀,可将残留在吸管中的 1-2 滴菌悬液转接至固体培养基上。
5、将液体试管和斜面试管于推荐条件下静置培养,以液体培养结果为准。
三、甘油冻存管菌种打管复活操作步骤 
1、准备好推荐的菌种培养基(见《菌种说明书》)。
2、将冻存管下半部浸入 37℃温水中轻轻摇动,使冷冻状态的菌种悬液迅速融化。如果收到菌 种冻存管时菌悬液是融化状态则需要立即进行转接培养。 3、用 75%酒精棉擦拭冻存管表面进行消毒。
4、在生物安全柜中打开冻存管瓶盖,用无菌吸管吸取全部菌悬液,均匀涂布到 2 支试管斜面 或平板上。 
5、将斜面试管或平板于推荐条件下静置培养。对于生长缓慢的菌种应持续培养 2 周至更长的 时间。
四、注意事项 
1、 所有打管操作都需要无菌操作。需由专业微生物技术人员在相应防护设备中进行,生物危 害程度为三类的菌种应在生物安全柜中操作。 
2、 西林瓶和冻存管打开后需一次用完,不能留存。 
3、 菌种经过冻干保藏后,处于休眠状态,需要转接 2-3 代恢复活力。
4、 大部分冻干菌种会在培养几天后生长。有些菌种第一代恢复生长时会有较长的延迟期,培养此类菌种时需要将培养时间延长至正常培养时间的双倍时长,甚至 2 周至更长的时间。