基本信息
启动子: CMV
复制子: pUC
质粒大小: 7567bp
原核抗性: Amp
筛选标记: EGFP
克隆菌株: Stbl3
培养条件: 37度
质粒属性
载体宿主: 哺乳细胞,慢病毒
载体用途: 干扰沉默
基因种属:
基因类型: shRNA
原核抗性: Amp
真核抗性:
荧光蛋白: 绿色
质粒简介
EGFP is expressed from this plasmid as a marker, but it is not a fusion protein. EGFP and the shRNA oligo are flanked by LoxP sites. Cre causes both to be recombined out of the construct, turning off shRNA expression.
pSico shRNA cloning protocol