CCD-34Lu 拉丁名
(ATCC® CRL-1491™) 统一编号
Organism Homo sapiens, human
Tissue lung
Cell Type fibroblast
Product Format 提供形式 frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 生物安全等级 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease normal
Age 2.5 weeks
Gender female
Ethnicity Black
Applications 用途 The line was derived from normal lung tissue from a child who died of erythroblastosis (Rh incompatibility).
A normal skin line derived from the same patient is available as ATCC CRL-1497
Derivation The line was derived from normal lung tissue from a child who died of erythroblastosis (Rh incompatibility).
A normal skin line derived from the same patient is available as ATCC CRL-1497
Clinical Data The line was derived from normal lung tissue from a child who died of erythroblastosis (Rh incompatibility). A normal skin line derived from the same patient is available as ATCC CRL-1497 female Black 2.5 weeks
Comments 注释 The line was derived from normal lung tissue from a child who died of erythroblastosis (Rh incompatibility).
A normal skin line derived from the same patient is available as ATCC CRL-1497
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003.
To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium 培养基 Renewal: Twice per week Remove medium, add fresh 0.25% trypsin solution for 2 to 3 minutes, remove trypsin and let the culture sit at 37C for 5 to 10 minutes.
Add fresh medium, aspirate and dispense into new flasks. Subculture every 5 to 7 days.
Culture Conditions 培养条件
Temperature 培养物 : 37.0℃
STR Profile
Amelogenin: X CSF1PO: 11,13
D13S317: 9,13
D16S539: 8,13
D5S818: 12
D7S820: 10
THO1: 7,9
TPOX: 11 vWA: 15,16
Name of Depositor 寄存人 GP Maxwell