年限：Neonatal
运输方式：冻存运输
是否是肿瘤细胞：0
物种来源：人
数量：大量
细胞形态：其他
生长状态：贴壁生长
ATCC Number：PCS-110-010
规格：0.1ml Name: Primary Dermal Microvascular Endothelial Cells; Normal, Human, Neonatal
Biosafety Level:1
Shipped: frozen
Organism: Homo sapiens (human)
Source: Neonatal foreskin
Growth Properties: Adherent
Age: Neonatal
Morphology: Cobblestone appearance
Volume per Vial: 1 ml
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Related Products:Vascular Cell Basal Medium (ATCC PCS-100-030 )
Microvascular Endothelial Cell Growth Kit-BBE (ATCC PCS-110-040 )
Microvascular Endothelial Cell Growth Kit-VEGF (ATCC PCS-110-041 )
Dulbecco's Phosphate Buffered Saline (D-PBS) (ATCC 30-2200 )
Trypsin-EDTA for Primary Cells (ATCC PCS-999-003 )
Trypsin Neutralizing Solution (ATCC PCS-999-004 )
Gentamicin-Amphotericin B Solution (ATCC PCS-999-025 )
Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002 )
Phenol Red (ATCC PCS-999-001 )
Recomended Culture Conditions:See Product Information Sheet Comments:The cells are cryopreserved in the third passage to ensure the greatest purity of the cell population, coupled with high viability and plating efficiency.
A complete solution to propagate dermal microvascular cells in low serum (5% FBS) conditions with or without the addition of human recombinant VEGF. Use of the Endothelial Cell Growth Kit-VEGF will support a faster rate of proliferation because of the presence of several purified human recombinant (rh) growth factors (rh VEGF, rh EGF, rh FGF basic and rh IGF-1) combined with heparin and hydrocortisone. Use of the Microvascular Endothelial Cell Growth Kit-BBE, which contains Bovine Brain Extract (BBE), is recommended if a more "authentic," less defined culture medium is desired.
Applications:Drug screening, angiogenesis, oncology, inflammation, intercellular communication
Viability:≥ 50%
Population Doubling Capacity:≥ 15 in complete growth medium
Sterility Tests: Bacteria and Yeast: Negative
Mycoplasma: Negative

Testing: Hepatitis B: Negative
Hepatitis C: Negative
HIV-1 and HIV-2: Negative