生长状态:贴壁生长
ATCC Number:CRL-1336?
相关疾病:其他疾病
数量:大量
细胞形态:成纤维样
运输方式:冻存运输
年限:3 years
是否是肿瘤细胞:0
物种来源:人
器官来源:皮肤
规格:0.5mg Designations: Tep Be
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast
Source: Organ: skin
Disease: Cockayne syndrome
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Age: 3 years
Gender: male
Comments:The tissue donor suffered from Cockayne syndrome.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Remove spent medium, add fresh 0.25% trypsin for 3 to 5 minutes, remove trypsin and let the culture sit at 37C for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.