器官来源:肺
运输方式:冻存运输
细胞类型:成纤维细胞
细胞形态:成纤维样
是否是肿瘤细胞:0
物种来源:人
ATCC Number:CCL-191?
相关疾病:其他疾病
年限:48 years
数量:大量
生长状态:贴壁生长
规格:0.5mg Designations: LL 97A (AlMy)
Depositors: ?RG Crystal
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast
Source: Organ: lung
Disease: idiopathic pulmonary fibrosis
Cell Type: fibroblast
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 12
D13S317: 11,13
D16S539: 11,13
D5S818: 13
D7S820: 9,11
THO1: 6,9.3
TPOX: 10,11
vWA: 14,17
Cytogenetic Analysis:diploid; relatively unstable
Isoenzymes: G6PD, B
Age: 48 years
Gender: male
Ethnicity: Caucasian
Propagation: ATCC complete growth medium: Ham's F12K medium, 85%; fetal bovine serum, 15%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, rinse monolayer once with fresh 0.25% trypsin and let the culture sit for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.