器官来源:肺
ATCC Number:CCL-192?
相关疾病:正常
品系:Sprague-Dawley
年限:18 days gestation
运输方式:冻存运输
细胞形态:成纤维样
细胞类型:成纤维细胞
生长状态:贴壁生长
是否是肿瘤细胞:0
物种来源:大鼠
数量:大量
规格:0.5ml Designations: RFL-6
Depositors: ?ML Macy
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology:fibroblast
Source: Organ: lung
Strain: Sprague-Dawley
Disease: normal
Cell Type: fibroblast
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 18 days gestation
Propagation: ATCC complete growth medium: Ham's F12K medium, 80%; fetal bovine serum, 20%
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin for 3 to 5 minutes, remove trypsin and let the culture sit for 10 to 12 minutes at 37C. Add fresh medium, aspirate and dispense into new flasks. Subculture every 5 to 6 days.
Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%