是否是肿瘤细胞:0
物种来源:小鼠
数量:大量
生长状态:悬浮生长
ATCC Number:CRL-1644?
细胞形态:淋巴样
免疫类型:IgG1
运输方式:冻存运输
规格:100ml Designations: SJK-287-38
Depositors: ?D Korn
Isotype: IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against human DNA polymerase alpha
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Antigen Expression:H-2db
Comments:Mice were immunized with fraction VIII human DNA polymerase alpha.
Spleen cells were fused with NS-1 myeloma cells.
The antibody neutralizes polymerase alpha activity, but does not react with DNA polymerases beta and gamma.
Tested and found negative for ectromelia virus (mousepox).
Formerly ATCC TIB-179.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures are maintained by addition or replacement of fresh medium. Start new cultures at 1 X 10 exp5 viable cells/ml and maintain at or below 1 X 10 exp6 cells/ml.
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 959: Hu SZ, et al. Preparation and preliminary characterization of monoclonal antibodies against human DNA polymerase alpha. J. Biol. Chem. 257: 8386-8390, 1982. PubMed: 7085672